An in vivo, luciferase-based, Agrobacterium-infiltration assay …
An in vivo assay system for analyzing transient luciferase expression in tobacco leaves infused with Agrobacterium tumefaciens is described. The system makes use of A. tumefaciens harboring T-DNA vectors containing either an intron-containing firefly (Photinus pyralis) luciferase (EC 1.13.12.7) gene …
Measurement of Luciferase Rhythms - SpringerLink
2014年1月1日 · For experiments in continuous light or in light–dark cycles, we interleave the sample plates with three clear microtiter plates to permit light penetration to the central wells of the sample plates (see Note 7).
Convenient site-selective protein coupling from bacterial raw …
2023年1月1日 · Luciferin conversion activity assays of ybbR-luciferase (ybbR-Luc) coupled by Sfp onto TMV Lys-PEG-CoA adsorbed in microtiter plate wells, and corresponding control samples. Luciferase sources: purified ybbR-Luc (top) or bacterial cell lysates (bottom).
High‐throughput protoplast transactivation (PTA) system for …
2011年7月12日 · For efficient screening, collections of TF expression vector DNAs are arrayed and stored in 96-well microtiter plates and can be combined with the Prom:LUC constructs of choice.
Three ethylene‐responsive transcription factors in tobacco with ...
2001年12月25日 · Chimeric promoters containing two copies of the GCC fragment or mGCC fragment upstream of a minimal 35S promoter (−46 to +8) of cauliflower mosaic virus (CaMV) were placed in front of a reporter gene for luciferase (LUC) from firefly and the polyadenylation signal of a gene for nopaline synthase (Nos).
Abstract. The properties of the firefly luciferase (LUC) make it a very good non-destructive reporter to quantify and image transgene promoter activity in plants. The short half-life of the LUC mRNA and protein, and the very limited regeneration of the LUC protein after reacting with luciferin, enables monitoring of changes in gene activity
A transcriptional repressor BrDof2.4 regulates protease genes …
2021年11月1日 · The transcriptional ability of BrDof2.4 was demonstrated by the ratio of luciferase (LUC) to renilla luciferase (REN). The LUC/REN ratio of the 62SK-BD vector (negative control) was used as a calibrator (set as 1).
Improved dual luciferase reporter (DLR) assay to determine the …
2021年1月1日 · We developed a binary vector co-expressing firefly luciferase (FF) and Renilla luciferase (REN) to detect protein stability in response to different stimuli, and verified the functionality of the vector. The StrigoQuant-like reporter expressing FF and REN in one transcript is a sensitive tool for detecting protein abundance in different ...
A NanoLuc-Based Transactivation Assay in Plants
2023年8月5日 · This ATP-independent luciferase is used with furanylmethyl-deoxy-coelenterazine (named furimazine), a more stable substrate compared with the substrate of other luciferases. NanoLuc only requires O 2 and furimazine to produce a …
NtMYB12 requires for competition between flavonol and …
2023年2月8日 · Biochemical evidences of dual-luciferase activity and chromatin immunoprecipitation assay supported that MYB12 directly bound to promoters of NtFLS, NtLAR, and NtDFR that were cloned by genome walking assay, and activated NtFLS and NtLAR expression but repressed NtDFR expression.