Functionalized graphene grids with various charges for single
2022年11月7日 · By applying onto three different types of macromolecules, we demonstrate that the high-yield charged graphene grids bring macromolecules away from the AWI and enable adjustable particle ...
Developing Graphene Grids for Cryoelectron Microscopy
2022年7月12日 · In this review, various advantages of graphene grids are discussed by comparing them with conventional holey carbon grids. In addition, to form an overall outline of the current development of graphene grids for cryo-EM, the recent progress in graphene film functionalization and the preparation of high-yield and clean graphene grids are discussed.
Functionalized graphene-oxide grids enable high-resolution cryo …
2024年3月12日 · Here we present a straightforward and reproducible method for determining structures of the SNF2h-nucleosome complex using graphene-oxide (GO) grids functionalized with negatively charged DNA...
Epoxidized graphene grid for highly efficient high-resolution …
2023年2月8日 · We have successfully achieved a novel oxidative modification of graphene using photoactivated ClO 2· as a mild oxidant and confirmed the oxidized graphene grid is storable with its...
Developing Graphene Grids for Cryoelectron Microscopy
2022年7月13日 · With graphene grids, protein particles can adsorb at the graphene–water interface layer, thereby preventing protein particles from diffusing to the air–water interface (Figure 1B), which can address the issues of the air–water interface–induced preferred orientation, denaturation, and dissociation effects (D'Imprima et al., 2019; Fan et ...
Developing Graphene Grids for Cryoelectron Microscopy
2022年7月13日 · Here, the various advantages of graphene grids over conventional holey carbon film grids, functionalization of graphene support films, production methods of graphene grids, and origins of pristine graphene contamination are reviewed and discussed.
Dual-Affinity Graphene Sheets for High-Resolution Cryo-Electron ...
2023年4月3日 · This polymer-free and transfer-free fabrication method for graphene grids effectively preserves the intactness and cleanliness of the graphene sheet. Next, we modified the graphene grids using NTA–Ni 2+ and PEG–biotin ligands, extensively applied in the affinity purification of macromolecules tagged with His 6 -tag and Avi-tag, respectively.
Streamlined production of graphene-supported grids enhances …
2024年2月8日 · Utilization of graphene grids as a supporting film in cryo-EM sample preparation has been explored owing to its potential to amplify sample concentration, minimize ice thickness, and provide a conducive environment for analyzing intricate protein complexes.
The use of graphene oxide (GO) has been shown to improve sample dispersion for single particle cryo-EM data collection. There are many different published methods on how to fabricate GO grids, with each method having its own pros and cons.
Simplified Approach for Preparing Graphene Oxide TEM Grids for …
2021年1月29日 · In this manuscript, we report the application of graphene oxide (GO) in the preparation of cryo-electron microscopy (cryo-EM) and transmission electron microscopy (TEM) grids. We treated GO with water and organic solvents, such as, methanol, ethanol and isopropanol separately to isolate significantly large GO monolayer flake to fabricate the ...