Engineered phage with antibacterial CRISPR–Cas selectively reduce
2023年5月4日 · Selected phages were engineered with tail fibers and CRISPR–Cas machinery to specifically target E. coli. We show that engineered phages target bacteria in biofilms, reduce the emergence of...
Bacteriophages and phage-delivered CRISPR-Cas system as …
2022年1月1日 · One effective way to deliver the CRISPR-Cas system is via engineered phage-based vectors, which can also be considered a phage-derived antimicrobial therapy [8]. In this article, we review recent advances in phage therapy and the CRISPR-Cas system as antimicrobial treatments as well as RCTs of bacteriophage therapy.
Bacteriophages suppress CRISPR–Cas immunity using RNA-based …
2023年10月18日 · Many bacteria use CRISPR–Cas systems to combat mobile genetic elements, such as bacteriophages and plasmids 1. In turn, these invasive elements have evolved anti-CRISPR proteins to block host...
Anti-CRISPR proteins trigger a burst of CRISPR-Cas9 expression …
2024年3月26日 · Here, we show that phage-encoded anti-CRISPR proteins relieve Cas9 auto-repression and trigger a rapid increase in CRISPR-Cas levels during a single phage infective cycle. As a result, fewer cells succumb to lysis, leading to a striking survival benefit after multiple rounds of infection.
CRISPR-CasΦ from huge phages is a hypercompact genome editor
2020年7月17日 · CRISPR-Cas systems are found widely in prokaryotes, where they provide adaptive immunity against virus infection and plasmid transformation. We describe a minimal functional CRISPR-Cas system, comprising a single ~70-kilodalton protein, CasΦ, and a CRISPR array, encoded exclusively in the genomes of huge bacteriophages.
Anti-CRISPR Phages Cooperate to Overcome CRISPR-Cas Immunity
Some phages encode anti-CRISPR (acr) genes, which antagonize bacterial CRISPR-Cas immune systems by binding components of its machinery, but it is less clear how deployment of these acr genes impacts phage replication and epidemiology. Here, we demonstrate that bacteria with CRISPR-Cas resistance are still partially immune to Acr-encoding phage.
Broad-spectrum CRISPR-Cas13a enables efficient phage genome …
2022年10月31日 · Using a two-step phage-editing and enrichment method, we achieved seven markerless genome edits in three diverse phages with 100% efficiency, including edits as large as multi-gene deletions and as...
Phage Genetic Engineering Using CRISPR–Cas Systems - PMC
Recent reports have demonstrated that CRISPR–Cas systems, specifically the three major types (I, II, and III), can be harnessed to genetically engineer phages that infect diverse hosts. Here, the mechanisms of each of these systems, specific strategies used, …
Cas1 mediates the interference stage in a phage-encoded
2024年3月11日 · CRISPR-Cas systems are prokaryotic adaptive immune systems against invading phages and other mobile genetic elements, which function in three stages: adaptation, expression and interference. Interestingly, phages were also found to encode CRISPR-Cas systems to antagonize their hosts and establish infection.
CRISPR/Cas9-Mediated Genome Editing of T4 Bacteriophage for …
2024年10月30日 · Herein, a simple, accurate, and high-throughput phage-based colorimetric sensing strategy was developed for antimicrobial susceptibility testing (AST). Taking advantage of the CRISPR/Cas9 system, the genome of the T4 phage was modularly engineered to carry lacZ-α (lacZa), a marker gene encoding the α-fragment of β-galactosidase (β-gal).