A mathematician’s guide to plasmids: an introduction to plasmid …
Plasmids, extrachromosomal DNA molecules commonly found in bacterial and archaeal cells, play an important role in bacterial genetics and evolution. Our understanding of plasmid biology has been furthered greatly by the development of mathematical models, and there are many questions about plasmids that models would be useful in answering.
Mathematical Models of Plasmid Population Dynamics
2021年11月4日 · In this review, we explore the myriad of mathematical models available in the field, with an emphasis on discussing different methodological approaches used to study plasmid dynamics in bacterial populations. We also present different approaches used to model horizontal transmission of plasmids between bacterial cells.
Plasmid DNA: Genetic Engineering, Cloning and Isolation
In addition, preclinical and clinical studies with plasmid DNA have returned promising results pointing to potential applications in gene therapy. In developing gene therapies for HER2-overexpressing cancers, plasmid DNA was used to encode anti-HER2 monoclonal antibodies, which reversed tumor growth in human muscle cell lines and mice models.
Machine learning-based prediction models to guide the ... - Cell …
2024年2月27日 · Li et al. develop powerful machine learning models for Cas9 variants. The user can utilize these prediction tools to select the most optimal Cas9 variant and corresponding gRNA in human cells while minimizing off-target effects.
Frontiers | Effects of buffer composition and plasmid toxicity on ...
2024年7月9日 · Additionally, we successfully performed long-term transfection of the murine 4T1 cell line using high-frequency nanosecond PEFs and confirmed its’ applicability in an in vivo model. The findings from the study can be applied to optimize electrotransfection conditions.
Guide for generating single-cell–derived knockout clones in …
2024年7月1日 · In this study, we provide a guide on single-guide RNA design, cloning single-guide RNA into plasmid vectors, single-cell isolation via transfection, and identification of knockout clones using next-generation sequencing.
Lentiviral vector packaging and producer cell lines yield titers ...
2024年9月12日 · To develop a packaging cell line, lentiviral packaging genes were stably integrated by random integration of linearized plasmid DNA. Then, to develop EGFP - and anti-CD19 chimeric antigen receptor-encoding producer cell lines, transfer plasmids were integrated by transposase-mediated integration.
A plasmid-chromosome crosstalk in multidrug resistant ... - Nature
4 天之前 · The authors describe a new crosstalk between a globally disseminated carbapenem resistance plasmid and clinical enterobacteria clones. This crosstalk provides a fitness advantage to the plasmid ...
Parallelized engineering of mutational models using ... - Cell Press
2024年1月22日 · We developed a workflow for engineering cellular models in parallel (Figure 1) to produce iPSC lines genetically differing only by CRISPR-induced mutations. Initially, we clone gRNA sequences into a PB plasmid vector to form a CRISPR library, with each resulting transposon containing a gRNA gene, inducible Cas9, and a puromycin resistance gene.
Stable Cell Line Generation - Danaher Life Sciences
Stable cell lines are cellular models that harbor genetic modifications to express specific proteins or biomolecules of interest. Generating stable cell lines involves a sequential series of events intended to produce a uniform population of cells that homogeneously express the transgene.