Single-cell RNA sequencing reveals the heterogeneity of liver
2020年4月28日 · Here, we dissect the diversity of LrICs by a comprehensive transcriptomic profiling using the unbiased single-cell RNA-sequencing (scRNA-seq). A total of 70, 706 of CD45 + immune cells from the...
单细胞转录组助力揭示心肌梗死后心脏免疫的动态变化
2022年2月23日 · 材料：小鼠心脏中的Cd45细胞. 期刊： 发表时间：2022年1月13日. 方法：欧易生物10x Genomics scRNA-seq. 研究背景. 心肌梗死（MI）是世界范围内发病率和死亡率的主要原因之一，其特点是免疫细胞的招募与激活。免疫细胞参与心肌梗死后最初的促炎过程以及后来的抗炎 ...
Single-cell sequencing of immune cells from anticitrullinated …
2021年8月17日 · Here we apply single-cell RNA sequencing (scRNA-seq) to characterize the cell composition, proportion, gene expression signature, and developmental trajectories of CD45 + cells in the...
IDEIS: a tool to identify PTPRC/CD45 isoforms from single-cell ...
2024年10月8日 · The expression of CD45 isoforms can be identified in scRNA-seq experiments using a method called CITE-seq. It is based on labeling cell surface proteins with specific antibodies conjugated with DNA oligonucleotides, which can be subsequently sequenced and matched with the corresponding cell transcriptomes ( 14 ).
Single-cell RNA sequencing reveals evolution of immune ... - Nature
2022年5月27日 · Temozolomide decreased the accumulation of myeloid-derived suppressor cells, whereas concomitant temozolomide irradiation increased intratumoral GranzymeB + CD8 + T cells but also increased CD4 +...
An immune cell atlas reveals the dynamics of human macrophage ...
2023年9月28日 · To explore the spatiotemporal dynamics of human Mf specification, we began with an unbiased approach by sorting all the immune cells (CD45 + cells) for single-cell RNA sequencing (scRNA-seq) analysis from 19 organs/tissues of 52 human embryos and fetuses. In total, we collected 367 prenatal samples spanning 19 time points encompassing ...
Enhanced annotation of CD45RA to distinguish T cell subsets in …
2023年11月6日 · In order to facilitate cell-type annotation in T cell scRNA-seq analysis, we employed machine learning and trained a CD 45 RA + / − classifier on single-cell mRNA count data annotated with known CD45RA antibody levels provided by cellular indexing of transcriptomes and epitopes sequencing data.
Single cell profiling of CD45 - BioMed Central
2022年11月4日 · In the current study, we profiled resident and infiltrating CD45 + cells in the intact and injured spinal cord at subacute and chronic stages using scRNA-seq to establish how the various immune cells change and respond to the evolving injury.
Landscape and Dynamics of Single Immune Cells in …
2019年10月31日 · Here we combined full-length and 3′ scRNA-seq technologies to obtain high-quality data for a large collection of CD45 + immune cells from various tissues in HCC patients. For the first time, cell composition, functional states, developmental trajectory, and cellular interactions of immune cells in HCC tumors, adjacent liver, hepatic lymph ...
Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted ... - Cell …
2020年4月16日 · Combined scRNA-seq analyses on the tumor microenvironment in colorectal cancer and murine tumor models identify distinct myeloid populations that convey differential sensitivity to CSF1R blockade and define concerted immune responses involving dendric cells and T cells upon anti-CD40 treatment.