Western Blotting - Bio-Rad
Industry-friendly gel documentation system that calibrates itself before every run. Select from Bio-Rad's western blotting systems, buffers, membranes, and immunodetection reagents and kits. Total solutions for your blotting workflow.
Types of Western Blotting Equipment (Cells & Power Supplies) - Bio-Rad
This section provides an overview of the various blotting equipment available from Bio-Rad for different transfer methods, such as tank blotting, semi-dry blotting, and microfiltration or dot-blotting.
Introduction to Western Blotting - Bio-Rad
Western blotting is a powerful technique that allows you to positively detect your proteins, estimate quantities, and determine their molecular weights. All from a starting mixture of proteins extracted from cells or tissues.
Western Blot Protocol - Bio-Rad
This video demonstrates SDS-PAGE separation of proteins using the Bio-Rad Comparative Proteomics Kit II: Western Blot Module. Assembly of the blotting sandwich and electroblotting are shown along with the steps for protein detection using a colorimetric assay.
Western Blotting Workflow Products - Bio-Rad
Explore Bio-Rad's complete solutions for your western blotting workflow - gels, electrophoresis chambers, western blotting transfer and imaging systems, buffers, membranes, and immunodetection reagents and kits.
All Courses - Bio-Rad Academy
Take a deep dive into advanced western blotting sample preparation. In this course you will learn about: protein sources, cell lysis, sample preparation buffers, and reduction.
Fundamentals of Western Blotting Sample Preparation - Bio-Rad …
Learn how western blots are used in biological research, experimental design considerations before you begin your project, and reagents and methods for good sample preparation.
Bio-Rad Laboratories, Inc. Life Science Group General Protocol for Western Blotting Protein separation by gel electrophoresis 1. Load equal amounts of protein (20 μg) into the wells of a mini (8.6 x 6.7 cm) or midi (13.3 x 8.7 cm) format SDS-PAGE gel, along with molecular weight markers. 2. Run the gel for 5 min at 50 V. 3.
Western Blot - What is Western Blotting? - Bio-Rad
Western blotting, also known as immunoblotting or protein blotting, is a core technique in cell and molecular biology. In most basic terms, it is used to detect the presence of a specific protein in a complex mixture extracted from cells or tissue.
Fundamentals of Western Blotting Image Acquisition - Bio-Rad …
In the fundamentals of western blotting series. Learn about imaging technologies, chemiluminescence and fluorescence detection, and how to optimize to get the best sensitivity.
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