polymerase chain reaction / PCR | Learn Science at Scitable - Nature
Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. PCR is very precise and can be used to amplify, or copy, a specific DNA target from a ...
The Biotechnology Revolution: PCR and Cloning Expressed Genes
1970年6月27日 · The cloning of expressed genes and the polymerase chain reaction (PCR), two biotechnological breakthroughs of the 1970s and 1980s, continue to play significant roles in science today. Both ...
Design and in silico validation of polymerase chain reaction
2021年6月15日 · Accurate designing of polymerase chain reaction (PCR) primers targeting conserved segments in viral genomes is desirable for preventing false-negative results and decreasing the need for ...
Scientists Can Make Copies of a Gene through PCR
In most cases, the polymerase chain reaction, ... At this point, the DNA polymerase begins making a new DNA strand by attaching to the primers and then adding dNTPs to the template strand, thereby ...
PCR-based techniques - Latest research and news - Nature
2024年11月16日 · PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...
Reverse transcription polymerase chain reaction - Nature
2024年12月28日 · Reverse transcription polymerase chain reaction (RTPCR) is a technique to detect or quantify RNA. It uses a reverse transcriptase enzyme to convert RNA to DNA, followed by PCR to amplify the DNA.
Mirror-image polymerase chain reaction | Cell Discovery - Nature
2017年10月17日 · We show that the d-polymerase is able to amplify a 120-bp l-DNA sequence coding for the Escherichia coli 5S ribosomal RNA gene rrfB by mirror-image polymerase chain reaction, and that both the ...
Diagnostics for SARS-CoV-2 infections - Nature Materials
2021年2月15日 · The first step in managing COVID-19 is the rapid and accurate detection of SARS-CoV-2 enabled by real-time reverse transcription–polymerase chain reaction (RT–PCR) 7.RT–PCR detects SARS-CoV ...
Polymerase chain reaction primers miss half of rRNA microbial
2009年8月20日 · The rRNA approach is the principal tool to study microbial diversity, but it has important biases. These include polymerase chain reaction (PCR) primers bias, and relative inefficiency of DNA ...
Ultrafast photonic PCR | Light: Science & Applications - Nature
2015年7月31日 · A team in the USA has realized an ultrafast polymerase chain reaction (PCR) method that uses plasmonic photothermal heating. The PCR is a powerful technique that is widely used in many fields ...