Imine reductase - Wikipedia
An imine reductase (IRED) is an enzyme that reduces imines to amines. [1] [2] This family of enzymes is employed in the industrial production of amine-containing pharmaceuticals. [3] The IRED enzymes that are found to catalyze both imine formation and imine reduction are called reductive aminases (RedAms).
Discovery of an Imine Reductase for Reductive Amination of …
2023年1月20日 · Herein, we report the direct synthesis of a wide range of sterically demanding secondary amines, including several important active pharmaceutical ingredients and pharmaceutical intermediates, via reductive amination of carbonyl substrates and bulky amine nucleophiles employing imine reductases.
Enhancing the Imine Reductase Activity of a Promiscuous Glucose ...
2024年4月22日 · Imine reductases (IREDs) have been identified as an important class of biocatalysts to synthesize chiral amines with substantial promise for industrial application. Here, we report the promiscuous imine reductase activity of a glucose dehydrogenase (GDH).
Expanding the repertoire of imine reductases by mining divergent ...
1 天前 · Imine reductases (IREDs) have emerged as important members of the catalyst toolkit for the synthesis of chiral amine building blocks. IREDs (including the closely related subclass of reductive aminases [RedAms]) catalyze the NAD(P)H-dependent enantioselective reduction of imines and reductive amination of carbonyl compounds, allowing access to primary, secondary, and tertiary chiral amines. 1 ...
Characterization of imine reductases in reductive amination for …
2020年5月22日 · Imine reductases (IREDs) have shown great potential as catalysts for the asymmetric synthesis of industrially relevant chiral amines, but a limited understanding of sequence activity relationships makes rational engineering challenging.
Imine Reductases: Multifunctional Biocatalysts with Varying Active ...
2022年9月12日 · Imine reductase (IRED), a promising biocatalyst that was previously known to only catalyze asymmetric imine reduction (IR), was revealed to achieve direct asymmetric reductive amination (RA) of ketones with excess amines, …
Reductive aminations by imine reductases: from milligrams to tons
2022年4月7日 · The discovery of asymmetric reductive amination by imine reductase (IRED) and reductive aminase (RedAm) enzymes has served as the starting point for a new industrial approach to the production of chiral amines, leading from laboratory-scale milligram transformations to ton-scale reactions that are now described in the public domain.
Engineering imine reductases for industrial applications
2019年10月11日 · Imine reductases are promising catalysts, facilitating a direct stereoselective route to secondary amines. Now, protein engineering has created stable and efficient variants that allow their...
An Engineered Imine Reductase for Highly Diastereo- and ...
2024年8月8日 · Herein, we report a highly diastereo- and enantioselective biocatalytic approach for preparing a broad range of β-branched chiral amines starting from their corresponding racemic ketones. This involves a dynamic kinetic resolution-asymmetric reductive amination process catalyzed using only an imine reductase.
Imine reductases (IREDs) - ScienceDirect
2017年4月1日 · Imine reductases (IREDs) are NADPH-dependent oxidoreductases that catalyse the asymmetric reduction of prochiral imines to the corresponding amines [1, 2, 3]. The reduction of C N bonds constitutes a physiological reaction present in a number of biosynthetic pathways leading to a variety of metabolites including folate, siderophores and ...