Purine nucleoside phosphorylase - Wikipedia
Purine nucleoside phosphorylase, PNP, PNPase or inosine phosphorylase (EC 2.4.2.1) is an enzyme that in humans is encoded by the NP gene. [2] It catalyzes the chemical reaction purine nucleoside + phosphate ⇌ {\displaystyle \rightleftharpoons } purine + alpha-D-ribose 1-phosphate
Purine Nucleoside - an overview | ScienceDirect Topics
Purine nucleosides are critical class of heterocyclic compounds that are widely distributed in all living organisms as constituents of nucleic acids and signal molecules [3]. They display a wide range of promising biological activities [4].
Purine nucleoside phosphorylases: properties, functions, and clinical ...
2000年12月1日 · Purine nucleoside phosphorylase (PNP, E.C. 2.4.2.1) catalyzes the cleavage of the glycosidic bond of ribo- and deoxyribonucleosides, in the presence of inorganic orthophosphate (P i) as a second substrate, to generate the …
Purine Nucleoside - an overview | ScienceDirect Topics
The purine nucleoside, Adenosine-5′-Triphosphate (ATP), discovered by Karl Lohmann in 1929 (Langen and Hucho, 2008), is the principal molecular unit of currency of intracellular energy transfer (Knowles, 1980) and is ubiquitous in all cell types throughout all organisms.
Purine nucleosides: endogenous neuroprotectants in hypoxic brain
In this review, the coherence of purine nucleoside-related pathways and MAPK activation in the endogenous neuroprotective regulation of the nervous system's development and neuroplasticity under hypoxic stress will be discussed.
Purine nucleoside phosphorylase controls nicotinamide riboside ...
Herein, we investigated the role of purine nucleoside phosphorylase (PNP) in NR metabolism in mammalian cells. Using both PNP overexpression and genetic knockout, we show that after being imported into cells by members of the equilibrative nucleoside transporter family, NR is predominantly metabolized by PNP, resulting in nicotinamide (Nam ...
Purine Nucleoside Phosphorylase. 1. Structure−Function Studies
1997年9月30日 · To probe the catalytic mechanism of human purine nucleoside phosphorylase (PNP), 13 active-site mutants were constructed and characterized by steady-state kinetics. In addition, microtiter plate assays were developed for both the phosphorolytic and synthetic reactions and used to determine the kinetic parameters of each mutant.
Bacterial Purine Nucleoside Phosphorylases from Mesophilic and …
2024年8月27日 · Purine nucleoside phosphorylases (PNPs) (EC 2.4.2.1) are nucleic acid metabolism enzymes involved in the utilization and reutilization pathways of purine nucleotides. This type of enzyme catalyzes the reversible phosphorolysis of purine nucleosides, both naturally occurring and their modified analogs [ 1 , 2 , 3 ].
Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside …
2024年9月23日 · In this study, we report the first development of two engineered bifunctional fusion enzymes, created through the genetic fusion of purine nucleoside phosphorylase I (PNP I) and thymidine phosphorylase (TP) from Thermus thermophilus.
Nucleoside-based anticancer drugs: Mechanism of action and …
2023年9月1日 · Nucleoside-based drugs, recognized as purine or pyrimidine analogs, have been potent therapeutic agents since their introduction in 1950, deployed widely in the treatment of diverse diseases such as cancers, myelodysplastic …