UltraMarathonRT® RT-qPCR Kit offers unmatched data reliability and sensitivity for gene expression studies, streamlining workflows and preserving RNA integrity.
In brief, two complementary single stranded oligonucleotides (one strand consisting of a 5ˊ-ATTG overhang plus 20-nt ... according to the manufacturer’s protocol. PCR was performed by using ...
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China School of Physical Science and ...
Polymerase chain reaction (PCR) was performed using an AllTaq PCR Core Kit (QUIAGEN). Samples were mixed with 0.5 X AllTaq PCR Buffer, 5 nM template strand, 0.25 μM primers, 200 μM of each dNTP, 2 X ...
PCR-based techniques are methods that rely on the polymerase chain reaction (PCR) to amplify stretches of DNA by creating many identical or near-identical copies. For example, PCR amplification ...
Polymerase chain reaction (PCR) was performed using an AllTaq PCR Core Kit (QUIAGEN). Samples were mixed with 0.5 X AllTaq PCR Buffer, 5 nM template strand, 0.25 µM primers, 200 µM of each dNTP, 2 X ...