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分离纯化蛋白质的方法及原理
5、盐析与盐溶 。原理:低浓度时,中性盐可以增加蛋白质溶解度这种现象称为盐溶.当离子强度增加,足够高时,例如饱和或半饱和程度,很多蛋白质可以从水中沉淀出来,这种现象称为盐析。 (三)根据电荷不同 6、SDS-PAGE 原理:通过加热和SDS可以使 ...
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