After 2 hours, monocytes were isolated as described above for immunoblotting or immunofluorescence microscopy. For static adhesion assay, they were isolated 6 hours after VLDL injection. For NF-κB p65 ...
The short answer is a resounding — yes, and it really isn't that much work. If you're seriously considering staining your concrete, you may think the benefits far outweigh the disadvantages, but these ...
MmMYL3 (green) and CP (red) were detected by immunofluorescence staining with anti-Flag or anti-Myc abs ... (K and L) hMMES1 cells were treated with inhibitors of Rac1 (EHT1814) or Cdc42 (MLS-573151) ...
Biotin-conjugated anti-rabbit, anti-goat, or anti-mouse IgG (1:500 dilution) was used as a secondary and was detected by a StreptABCcomplex/HRP kit (DAKO). For immunofluorescence staining, specific ...