Whereas, all models are restricted to mice, lacking analysis of human samples or human intestinal organoids to prove the human relevance of these findings ... Therefore, this manuscript offers novel ...
Design We generated 42 lines of human and murine organoids from gastric and intestinal segments of both adult and fetal tissues ... No additional mice were used for this study. Mouse lines used and ...
LGR5, a Wnt target gene, is a marker for mouse intestinal stem cells. Wnt signals are required for the development of mouse intestinal organoids from single intestinal stem cells in culture.
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