PCR—the workhorse of modern molecular biology ... is even more complicated than DNA amplification because amounts of mRNA transcripts vary widely between cells. “Basically it is possible ...
In that respect it follows a similar model to Merck's takeover of AmpTec, another CDMO that uses a PCR platform to make mRNA drugs, for an undisclosed sum last year. Outsourcing services are on of ...
So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort has been spent defining the "correct" conditions that ...
This study provides important insights into the regulation of a retained intron in the mRNA coding for OGT, a process known to be regulated by the O-GlcNAc cycling system, and highlights the ...
However, the therapeutic effectiveness is limited by the poor translation uptake of naked mRNA. To circumvent this challenge, researchers from VIB, VUB, Ghent University, and eTheRNA ...
Gen-Probe is currently developing the APTIMA HPV Assay, targeting E6/E7 mRNA from 14 carcinogenic HPV genotypes. A prototype of this assay was evaluated recently in 536 women with histological ...