High-salt wash buffer: 500 mM NaCl, 0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl (pH 8) LiCl wash buffer: 0.25 M LiCl, 1% NP-40 (or IGEPAL CA-360), 1% DOC, 1 mM EDTA, 10 mM Tris-HCl (pH 8 ...

This valuable report describes the changing antiviral activity of IFIT1 across mammals and in response to distinct viruses, likely as a result of past arms races. One of the main strengths of the ...

Figure 2: Precipitation by spermidine of 40 μg/ml pBGS19Luxwt or Baker's yeast RNA in 10 mM Tris buffer at pH 8.0 with and without 600 mM NaCl. To eliminate compaction agent from the DNA pellet ...

With the state to begin handing out licenses to cannabis businesses next year, the Detroit Lakes City Council met for a work ...

Human leukocyte antigen (HLA) genotyping is critical when preparing for stem cell transplants. The procedure is crucial to ...

Soft Condensed Matter and Biophysics, Department of Physics and Debye Institute, Utrecht University, Utrecht, The Netherlands ...

(Fragments greater than 1 kb long are not transferred efficiently by capillary action.) The gel is then soaked in alkaline solution to denature the DNA, and then neutralized in Tris/NaCl solution. The ...

Imagine you could earn much of the gains on stocks but none of their losses. Mutual funds and exchange-traded funds that use options contracts to insure against losses while capturing some gains ...

Finally, place the binding column in a new 2 ml collection tube. 11. Pipette 100 µl of Elution Solution (Tris-EDTA buffer, pH 9.0) directly into the centre of the binding column and let sit at room ...

Portra / Getty Images A capital buffer is mandatory capital that financial institutions are required to hold in addition to other minimum capital requirements. Regulations targeting the creation ...

Autophagy adaptors undergo liquid-liquid phase separation, but it remains unclear how this contributes to selective ...