Each TaqMan probe consists of a single-stranded oligonucleotide that is complementary to a sequence within the target template. It has a fluorescent dye at its 5′ end, whose signal is stifled by ...

Expert Rev Mol Diagn. 2012;12(7):731-754. SYBR Green and dual-hybridization probes are often used for melting point analysis at the end of the amplification. qPCR presents a number of advantages ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

As K is dependent on factors like (1) the reporter dye used in the probe (2) sequence context ... Thus, the overall efficiency of RT– and real-time PCR in detecting the housekeeping gene in ...

Total RNA (1pg, 100pg, 10pg, 1pg) was purified from a breast tissue FFPE sample using the RNeasy FFPE Kit. Transcripts of the human MUC1 gene were amplified and detected using the QuantiFast Probe ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative ... control of possible carryover contamination. Promega's PCR Systems, enzymes and reagents are proven in PCR to ensure ...

Let us help you with your inquiries, brochures and pricing requirements Request A Quote Download PDF Copy Download Brochure The QuantStudio 6 and 7 Pro real-time PCR ...