1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

Particles moving close to a planar wall undergo a considerable decrease in velocity, because of the viscous forces of the underlying fluid. The previous studies on electrophoretic migration very close ...