1| Prepare a 40-ml 4.5% native acrylamide gel (using 1 to 1.5-mm spacers): Prerun the 'gel for 2 h at 10 mA. Large protein complexes greater than 1 MDa in mass are difficult to resolve and migrate ...

Prepare a 90% formamide, 8% acrylamide gel (1.5 mm thick); use 7.5 inch × 7.5 inch plates and 20-well comb (each lane is 0.5 cm wide). In a beaker, combine 10 ml 10× Tris-borate-EDTA buffer (TBE ...