Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions ...
1| Harvest exponentially growing cells 24 h before transfection by trypsinization and replate them at a density of 1 × 10 5 to 4 × 10 5 cells/cm 2 in 60-mm tissue culture dishes or in 12-well ...
Want to share this FREE webinar with a colleague? Fill out the form below and we'll send your colleague an invitation to the "Accelerating Cell Line Development From Transfection to Manufacturing" ...
Electroporation uses short electric pulses to create transient pores in a cell’s membrane, allowing researchers to deliver intracellular ... to discover an approach using the Neon NxT system that ...
Beginning at cloning or transfection requires more interaction between the VDL and you. Briefly, once cells have been transfected, an agar overlay is applied to the cells so that virus-producing cells ...