物理隔离是实现”一管式”CRISPR检测的最直接方法，常见的方式是通过特制的隔离介质将扩增和酶切反应固定于两个腔室中，扩增后再通过手动操作将酶切体系和扩增产物混合，但这种方式仍面临试剂固定稳定性、操作复杂等挑战。微流控芯片通过集成多个反应室于单一反应 ...

Professor Kevin J. Zwezdaryk and Chandler H. Monk discuss CRISPR and diagnostics, focusing on the development of a portable ...

2024年12月16日，生命科学学院刘亮教授团队在《Nucleic Acids Research》期刊上在线发表了题为“DNA target binding-induced pre-crRNA processing in type II and V CRISPR-Cas systems”的研究论文，首次揭示了靶标核酸的结合能够激活CRISPR-Cas系统效应蛋白对pre-crRNA的反式切割活性 ...

作者说，这些系统具有作为生物技术进行研发的巨大潜力。CRISPR-Cas系统已经转变了基因组编辑和其它生物技术；然而，这些RNA引导机制的更广泛的 ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

The core components of CRISPR-based genome-editing therapies are bacterial proteins called nucleases that can stimulate ...

signaling the system to initiate gene silencing. "The 'R' in R-loop stands for RNA. All DNA-binding CRISPR-Cas systems use this structure to probe the DNA sequence and identify the correct target ...

Type V CRISPR-Cas Cpf1 endonuclease employs a unique mechanism for crRNA-mediated target recognition ... We have investigated the binding of anti-CRISPR proteins to the crRNA-bound type I-F Csy ...

为了解决CRISPR-Cas基因组编辑中的一个根本性局限，研究人员研发了新型的经设计的Cas9变体，后者几乎消除了对一个被称为PAM的原间隔序列相邻基序 ...

Yet others, called base editors, change one letter of the DNA code to another. So why do we call it CRISPR? Cas proteins are used by bacteria to destroy viral DNA. They add bits of viral DNA to ...

Researchers have been working to find CRISPR/Cas nucleases with greater specificity ... team used a GFP activation expression reporting system to screen 13 Cas12b nucleases. Interestingly, one ...