大家都知道，CRISPR/Cas9基因编辑技术在高效获得基因敲除突变（knockout）方面是非常有效的，但是想要通过同源重组修复途径 ...

实现更特异、更高效的基因敲入（knockin）！ CRISPR/Cas9和其它基因编辑工具已经被成功应用于获得基因敲除突变（knockout），但众多事实证明在基因敲入上的应用有很大的难度。基因敲入所面临的主要难题是修复模板的制备和如何与Cas9-sgRNA核糖核蛋白复合物共导入 ...

A fresh wave of gene-editing therapies is surging to the fore — even as the field wrestles with the challenge of getting the first generation of expensive and complex CRISPR treatments to the ...

CRISPR-Cas9技术因其高效的基因编辑能力成为功能基因组学的重要工具。然而，传统CRISPR筛选在复杂的体内模型（in vivo）中常面临瓶颈效应与生物异质 ...

Researchers have uncovered a serious side effect of using the CRISPR-Cas gene scissors. A molecule designed to make the process more efficient destroys parts of the genome. Genome editing with ...

CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

CRISPR-Cas tools allow researchers to modify individual building blocks of genetic material in a precise and targeted manner. Gene therapies based on such gene editing are already being used to ...