Figure 3: Before emPCR and sequencing, Genome Sequencer process ... Prepare a 90% formamide, 8% acrylamide gel (1.5 mm thick); use 7.5 inch × 7.5 inch plates and 20-well comb (each lane is ...

The reason, Drmanac will tell you, is also quite simple. He hated acrylamide gels. “I've never run a sequencing gel in my life,” he says—quite an admission from someone who has made a career ...